6FXX

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Fe2+, Mn2+, UDP-Gal, Hg2+ Soak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0059
Spacegroup name	C 2 2 21
Unit cell lengths	98.679, 100.330, 225.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.340 - 3.000
R-factor	0.2203
Rwork	0.219
R-free	0.24750
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.208	0.849
Rpim	0.060	0.251
Number of reflections	22895	3654
<I/σ(I)>	13.9	3.9
Completeness [%]	99.9	99.9
Redundancy	23.5	22.8
CC(1/2)	0.999	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 0.5 mM FeCl2, 0.5 mM MnCl2, 1 mM UDP-Glc