6FXS

Structure of Trypanosoma brucei type B ribose 5-phosphate isomerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.912
Spacegroup name	P 41 2 2
Unit cell lengths	97.800, 97.800, 84.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.560 - 2.010
R-factor	0.16565
Rwork	0.164
R-free	0.19774
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k7o
RMSD bond length	0.021
RMSD bond angle	2.102
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.560	2.130
High resolution limit [Å]	2.010	2.010
Number of reflections	27783
<I/σ(I)>	22.12	4.45
Completeness [%]	99.8	99.5
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	100mM hepes pH7.5; 1.26M NH4SO4