6FXL
Structure of Trypanosoma cruzi type B ribose 5-phosphate isomerase (TcRpiB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 171.160, 175.350, 93.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.580 - 1.960 |
| R-factor | 0.16208 |
| Rwork | 0.161 |
| R-free | 0.18387 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k7o |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.369 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 99426 | 15492 |
| <I/σ(I)> | 26.65 | 6.17 |
| Completeness [%] | 98.8 | 96 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277.15 | 100mM phosphate citrate pH4.2; 1.6M NaH2PO4/0.4M K2HPO4 |
