6FXD
Crystal structure of MupZ from Pseudomonas fluorescens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 42.330, 80.090, 78.820 |
Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
Resolution | 31.233 - 1.450 |
R-factor | 0.1624 |
Rwork | 0.161 |
R-free | 0.19470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dpo |
RMSD bond length | 0.006 |
RMSD bond angle | 0.781 |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.233 | 1.502 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.041 | |
Rmeas | 0.049 | |
Rpim | 0.027 | |
Number of reflections | 45031 | |
<I/σ(I)> | 13.21 | |
Completeness [%] | 98.6 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM amino acids II, 0.1 M MOPSO, Bis-Tris pH 6.5 and 25% w/v PEG 4000, 40% w/v 1,2,6-hexanetriol |