6FWW

GFP/KKK. A redesigned GFP with improved solubility

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97908
Spacegroup name	P 21 21 21
Unit cell lengths	31.814, 60.861, 106.401
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.200 - 1.131
R-factor	0.1849
Rwork	0.184
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gfl
RMSD bond length	0.013
RMSD bond angle	1.672
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (1.7.3-928)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.861	60.861	1.135
High resolution limit [Å]	1.131	5.250	1.131
Rmerge	0.092	0.047	0.800
Rmeas	0.105	0.054	0.931
Rpim	0.050	0.027	0.458
Number of reflections	77655	901	747
<I/σ(I)>	8
Completeness [%]	99.5	99.9	92.6
Redundancy	4.2	4	3.9
CC(1/2)	0.997	0.996	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M MMT buffer, 25 % PEG 1500, pH 4.0