6FWU

Crystal structure of human wild type beta-1,4-galactosyltransferase-1 (B4GalT1) in apo-closed dimeric form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 31 2 1
Unit cell lengths	60.217, 60.217, 229.368
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.472 - 2.350
R-factor	0.1983
Rwork	0.195
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ee3
RMSD bond length	0.002
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.472	2.434
High resolution limit [Å]	2.350	2.350
Rmerge	0.323	1.655
Rmeas	0.342	1.750
Rpim	0.112	0.562
Number of reflections	21046	2045
<I/σ(I)>	10.48	1.78
Completeness [%]	99.8	99.51
Redundancy	9.3	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium Nitrate, 20% (w/v) PEG 3350