6FWS

Structure of DinG in complex with ssDNA and ADPBeF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	109.771, 119.751, 126.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.370 - 2.500
R-factor	0.221
Rwork	0.219
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.080
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.370	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	58152
<I/σ(I)>	9.6
Completeness [%]	99.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M MgCl2, 0.1M HEPES (pH 7.0), and 15% PEG4000