6FW2

Crystal Structure of human mARC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	61.133, 74.818, 110.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.570 - 1.780
R-factor	0.1714
Rwork	0.169
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.570	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.084	0.418
Rmeas	0.091	0.473
Rpim	0.034	0.217
Number of reflections	45538	664
<I/σ(I)>	10.9
Completeness [%]	91.9	23.9
Redundancy	6.8	4.2
CC(1/2)	0.995	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Bis-TRIS propane, Na2MoO4, PEG3350