6FVS
The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - sTeLIC in complex with Barium ions (Ba2+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2016-12-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 2.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.840, 112.110, 144.080 |
| Unit cell angles | 90.00, 112.79, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.22600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 52251 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 292 | 150 mM MaCl2 20 mM tris 8.0 35% PEG 200 |
