6FVO
Mutant DNA polymerase sliding clamp from Mycobacterium tuberculosis with bound P7 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.488, 81.064, 90.015 |
| Unit cell angles | 68.64, 88.94, 62.56 |
Refinement procedure
| Resolution | 58.642 - 2.689 |
| R-factor | 0.2122 |
| Rwork | 0.210 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tr7 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.642 | 68.509 | 2.995 |
| High resolution limit [Å] | 8.531 | 2.689 | |
| Rmerge | 0.046 | 0.889 | |
| Rmeas | 0.054 | 1.046 | |
| Rpim | 0.028 | 0.546 | |
| Number of reflections | 1558 | 1558 | |
| <I/σ(I)> | 22 | 1.4 | |
| Completeness [%] | 99.1 | 49.1 | |
| Redundancy | 3.8 | 3.6 | |
| CC(1/2) | 0.997 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | Acetate de Na 3.2M pH 6.9 18% PEG 3350 (1microliter)+ Hampton Research PEG Ion kit B8 (1microliter): Magnesium formate dihydrate 0.2M; 20% PEG 3350; pH 7 |
