6FVO

Mutant DNA polymerase sliding clamp from Mycobacterium tuberculosis with bound P7 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	78.488, 81.064, 90.015
Unit cell angles	68.64, 88.94, 62.56

Refinement procedure

Resolution	58.642 - 2.689
R-factor	0.2122
Rwork	0.210
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tr7
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.642	68.509	2.995
High resolution limit [Å]		8.531	2.689
Rmerge		0.046	0.889
Rmeas		0.054	1.046
Rpim		0.028	0.546
Number of reflections		1558	1558
<I/σ(I)>		22	1.4
Completeness [%]		99.1	49.1
Redundancy		3.8	3.6
CC(1/2)		0.997	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Acetate de Na 3.2M pH 6.9 18% PEG 3350 (1microliter)+ Hampton Research PEG Ion kit B8 (1microliter): Magnesium formate dihydrate 0.2M; 20% PEG 3350; pH 7