6FVN

DNA polymerase sliding clamp from Mycobacterium tuberculosis with bound P7 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	79.447, 126.761, 171.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	101.837 - 3.142
R-factor	0.2579
Rwork	0.256
R-free	0.29120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tr7
RMSD bond length	0.003
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	101.837	171.085	3.198
High resolution limit [Å]	3.142	8.532	3.143
Rmerge	0.378	0.047	3.178
Rmeas	0.393	0.049	3.314
Rpim	0.107	0.014	0.935
Number of reflections	27157	1687	153
<I/σ(I)>	9.7	42.1	1.2
Completeness [%]	92.9	99.9	12.8
Redundancy	13.3	12.2	12.4
CC(1/2)	0.993	0.999	0.309

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		278	Acetate de Na 3.2M pH 6.9 18% PEG 3350 (1microliter)+ Hampton Research PEG Ion kit A2 (1microliter): 0.2M potassium fluoride, 20% PEG 3350 pH 7.3