6FV7

Dimer structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	72.600, 116.500, 142.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.884 - 3.700
R-factor	0.3288
Rwork	0.327
R-free	0.35920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fv6
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.830
High resolution limit [Å]	3.700	3.700
Rmerge	0.233	1.109
Rmeas	0.257
Rpim	0.107	0.482
Number of reflections	13317	1314
<I/σ(I)>	5	1.5
Completeness [%]	99.7	99.8
Redundancy	5.7	6
CC(1/2)	0.996	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	295	0.1 M Sodium chloride 0.1 M Magnesium chloride 0.1 M MES 40 % PEG 200 (v/v)