6FV6
Monomer structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 149.801, 149.801, 63.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.972 - 3.800 |
| R-factor | 0.2996 |
| Rwork | 0.298 |
| R-free | 0.32600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mlb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.592 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.145 | 1.778 |
| Rmeas | 0.156 | |
| Rpim | 0.057 | 0.664 |
| Number of reflections | 7452 | 739 |
| <I/σ(I)> | 9.6 | 1.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 8 |
| CC(1/2) | 0.999 | 0.616 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291 | 0.1 M Tris 1.2 M ammonium sulphate |
