6FUX

Structure of aminoglycoside phosphotransferase APH(3'')-Id from Streptomyces rimosus ATCC10970 in complex with ADP and streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.970, 77.800, 78.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.300 - 1.650
R-factor	0.1699
Rwork	0.168
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fuc
RMSD bond length	0.021
RMSD bond angle	2.760
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.300	55.300	1.700
High resolution limit [Å]	1.650	50.000	1.650
Rmerge	0.138	0.026	0.651
Rmeas	0.151	0.030	0.710
Total number of observations	208148
Number of reflections	34969	1	2927
<I/σ(I)>	8.04	14.21	2.2
Completeness [%]	98.8	100	98.2
Redundancy	5.952	4	6.197
CC(1/2)	0.992		0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	0.1M MES monohydrate; 12% PEG20000