6FUV

Structure of a manno-oligosaccharide specific solute binding protein, BlMnBP2 from Bifidobacterium animalis subsp. lactis ATCC 27673 in complex with mannotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	52.170, 64.590, 133.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.585 - 2.001
R-factor	0.1819
Rwork	0.178
R-free	0.25060
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.585	40.585	2.073
High resolution limit [Å]	2.000	10.000	2.000
Rmerge	0.229	0.044	1.137
Rmeas		0.054
Rpim	0.150		0.776
Number of reflections	28205	289	1657
<I/σ(I)>	4.45	18.34	0.64
Completeness [%]	90.3	95.1	53.66
Redundancy	3.027	2.92	2.099
CC(1/2)	0.968	0.996	0.388

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	278	Reservoir: 0.1 M MES pH 5.5, 0.05 M Zn-acetate and 18% PEG 8000 Protein 15 mg/ml in 20 mM MES pH 6.5, 150 mM NaCl + 1 mM Mannotriose. Drops ratio 1/1