6FTV
X-ray structure of human heavy chain ferritin in complex with NAMI A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.380, 182.380, 182.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 105.300 - 1.580 |
| R-factor | 0.14431 |
| Rwork | 0.142 |
| R-free | 0.18307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n27 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.133 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.300 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.068 | |
| Number of reflections | 35972 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M MgCl2, 0.1 M bicine pH 9.0 |
