6FTF
Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.09 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.846, 89.066, 45.634 |
Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
Resolution | 40.400 - 1.092 |
R-factor | 0.150566354651 |
Rwork | 0.150 |
R-free | 0.15986 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6flo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.400 | 1.131 |
High resolution limit [Å] | 1.090 | 1.092 |
Number of reflections | 215190 | |
<I/σ(I)> | 11.28 | |
Completeness [%] | 81.3 | |
Redundancy | 3.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | Elution buffer (Size Exclusion): 50 mM Hepes pH 7.5, 50 mM NaCl, 1 mM DMSO Crystallization buffer: 17% PEG 6.000, 0.2 M calcium acetate |