6FSK

F194Y mutant of the Dye-decolorizing peroxidase (DYP) from Pleurotus ostreatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	110
Detector technology	PIXEL
Collection date	2015-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976252
Spacegroup name	P 21 21 21
Unit cell lengths	62.717, 117.575, 140.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.220 - 1.560
R-factor	0.16386
Rwork	0.162
R-free	0.19885
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afv
RMSD bond length	0.021
RMSD bond angle	2.041
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.220	1.640
High resolution limit [Å]	1.560	1.560
Rmerge	0.065	0.514
Rmeas	0.083	0.649
Rpim	0.051	0.392
Number of reflections	144926	20996
<I/σ(I)>	12.7	2.3
Completeness [%]	97.3	97.4
Redundancy	4.5	4.6
CC(1/2)	0.997	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.75	295	0.2M MES pH=5.75 25%PEG 2000 MME