6FS0
INDUCED MYELOID LEUKEMIA CELL DIFFERENTIATION PROTEIN FABCOMPLEX IN COMPLEX WITH AZD5991
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-10 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.200, 42.050, 107.290 |
| Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
| Resolution | 28.150 - 2.250 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 2.320 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 29214 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1M sodium acetate-HCL pH 4.6 18%W/V PEG-8000 0.2M calcium acetate |
