6FRN
Structure of F420H2 oxidase (FprA) co-crystallized with 10mM Tb-Xo4 and calcium chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97662 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.660, 144.970, 74.210 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 25.480 - 1.740 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.18900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.840 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rpim | 0.040 | 0.458 |
| Number of reflections | 156147 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.9 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 28% PEG 400 and 200 mM calcium chloride in 100 mM Hepes pH7.5 |
