6FQ1

Crystal structure of the RRM12 domain of IMP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	30.130, 41.580, 72.490
Unit cell angles	92.01, 99.91, 108.94

Refinement procedure

Resolution	19.440 - 1.310
R-factor	0.1419
Rwork	0.140
R-free	0.17450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e44
RMSD bond length	0.009
RMSD bond angle	0.985
Data reduction software	MOSFLM (CCP4 7.0.050)
Data scaling software	SCALA (CCP4 7.0.050)
Phasing software	PHASER (CCP4 7.0.050)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.380
High resolution limit [Å]	1.310	1.310
Rmerge	0.044	0.423
Rmeas	0.053	0.505
Rpim	0.029	0.272
Number of reflections	75254	10684
<I/σ(I)>	12.3	2.8
Completeness [%]	95.1	92.4
Redundancy	3.2	3.2
CC(1/2)	0.999	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.2 M KNO3, 20% PEG3350