6FPZ

Inter-alpha-inhibitor heavy chain 1, D298A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 42
Unit cell lengths	159.700, 159.700, 65.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.410 - 2.200
R-factor	0.2184
Rwork	0.217
R-free	0.24760
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.410	71.410	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.066	0.025	0.498
Rmeas	0.077	0.030	0.591
Rpim	0.039	0.015	0.310
Total number of observations	312057
Number of reflections	83846	1019	6040
<I/σ(I)>	10.9
Completeness [%]	99.2	99.5	97.6
Redundancy	3.7	3.9	3.1
CC(1/2)	0.996	0.988	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100mM HEPES, pH 7.5, 100mM Sodium acetate, 10% PEG8K, 20% Glycerol