6FPQ

Structure of S. pombe Mmi1 in complex with 7-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 65
Unit cell lengths	77.406, 77.406, 66.211
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.100 - 1.420
R-factor	0.1442
Rwork	0.143
R-free	0.16780
RMSD bond length	0.017
RMSD bond angle	1.591
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.100	1.470
High resolution limit [Å]	1.420	1.420
Rmerge	0.084	1.160
Number of reflections	42567	4135
<I/σ(I)>	13.6	1.6
Completeness [%]	99.9	99.6
Redundancy	7.7
CC(1/2)	0.999	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% (v/v) Ethanol, 10mM Magnesium sulfate, 0.1M Tris pH 8