6FPL
TETR(D) E147A MUTANT IN COMPLEX WITH TETRACYCLINE AND MAGNESIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.020, 68.020, 179.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.590 - 1.602 |
| R-factor | 0.18772 |
| Rwork | 0.186 |
| R-free | 0.22093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2trt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.837 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.590 | 1.644 |
| High resolution limit [Å] | 1.602 | 1.602 |
| Rmerge | 0.047 | |
| Number of reflections | 28147 | 1959 |
| <I/σ(I)> | 31.95 | |
| Completeness [%] | 94.5 | 96 |
| Redundancy | 17.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | Precipitant 0.1M Na-citrat, 0.8M Li2SO4, 0.5M (NH4)2SO4, 0.1mL Protein (10.19mg/ml, 0.1M MES pH 6.5) + 0.1mL 2mM Tc + 0.001mL 3M MgCl2 1:4 Precipitant/Protein |
