6FPC
Structure of the PRO-PRO endopeptidase (PPEP-2) from Paenibacillus alvei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978570 |
Spacegroup name | P 43 |
Unit cell lengths | 84.900, 84.900, 113.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.900 - 1.750 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.20200 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 113.270 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.081 | 0.917 |
Number of reflections | 80890 | |
<I/σ(I)> | 14.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 2.2 M Ammonium Sulphate 0.2 M Cadmium Sulphate, 0.01 M Zinc Chloride pH 9.0 adjusted with Ammonium hydroxide |