6FPB

Crystal structure of anti-mTFP1 DARPin 1238_G01 in space group I4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99986
Spacegroup name	I 4
Unit cell lengths	66.224, 66.224, 77.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.827 - 1.617
R-factor	0.1526
Rwork	0.151
R-free	0.17460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ydy
RMSD bond length	0.009
RMSD bond angle	1.017
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2131: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.827	1.720
High resolution limit [Å]	1.617	1.617
Rmeas	0.066	0.935
Number of reflections	41912	4096
<I/σ(I)>	11.5	1.3
Completeness [%]	99.3	97.1
Redundancy	2.2	2.2
CC(1/2)	0.998	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	2 M NaCl, 10% PEG10000