6FP9
Crystal structure of anti-mTFP1 DARPin 1238_E11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.746, 91.597, 54.410 |
| Unit cell angles | 90.00, 116.93, 90.00 |
Refinement procedure
| Resolution | 48.510 - 2.100 |
| R-factor | 0.1643 |
| Rwork | 0.162 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ydy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.812 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2131: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.273 | 1.540 |
| Number of reflections | 25698 | 1843 |
| <I/σ(I)> | 7.7 | 2.1 |
| Completeness [%] | 99.4 | 85.8 |
| Redundancy | 3.7 | 3.1 |
| CC(1/2) | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M (NH4)2SO4, 0.1 M MES pH 6.5, 20% PEG8000 |
