6FP8
mTFP1/DARPin 1238_E11 complex in space group C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99986 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.953, 75.012, 48.295 |
Unit cell angles | 90.00, 90.73, 90.00 |
Refinement procedure
Resolution | 39.607 - 1.855 |
R-factor | 0.1498 |
Rwork | 0.148 |
R-free | 0.17640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hqk |
RMSD bond length | 0.012 |
RMSD bond angle | 1.301 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre_2131: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.607 | 1.970 |
High resolution limit [Å] | 1.855 | 1.855 |
Rmeas | 0.069 | 0.730 |
Number of reflections | 78111 | 12129 |
<I/σ(I)> | 11.2 | 1.7 |
Completeness [%] | 96.6 | 93.1 |
Redundancy | 2.4 | 2.3 |
CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 10% PEG4000, 20% Glycerol, and 0.02 M of L-glutamate, glycine, DL-alanine, L-lysin, DL-serin |