6FP5

Crystal structure of ZAD-domain of CG2712 protein from D.melanogaster

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9 and 1.28268
Spacegroup name	P 41 21 2
Unit cell lengths	97.650, 97.650, 48.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.050 - 2.000
R-factor	0.1613
Rwork	0.160
R-free	0.19230
Structure solution method	SAD
RMSD bond length	0.024
RMSD bond angle	2.269
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	97.650	97.650	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.182	0.058	0.759
Rmeas	0.195	0.063	0.809
Rpim	0.067	0.023	0.274
Total number of observations	119795
Number of reflections	16137	223	1173
<I/σ(I)>	9.5
Completeness [%]	98.9	96	99.7
Redundancy	7.4	6.9	7.8
CC(1/2)	0.993	0.990	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	0.1M MES pH 6.5; 1.3M Magnesium sulfate