6FOM

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 2 21 21
Unit cell lengths	62.129, 170.556, 197.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 2.900
R-factor	0.217
Rwork	0.214
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.009
RMSD bond angle	1.290
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	3.010
High resolution limit [Å]	2.900	2.900
Number of reflections	42339	0
<I/σ(I)>	7
Completeness [%]	89.1
Redundancy	5.5
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M sodium chloride 0.15 M ammonium sulfate 0.01 M MES sodium 19% (w/v) PEG1000