6FOG
X-ray structure of homo sapiens Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) in complex with inhibitor oxalate at 1.94A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-22 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.984, 116.679, 125.753 |
| Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
| Resolution | 85.530 - 1.940 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.20826 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.624 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmeas | 0.136 | 1.005 |
| Number of reflections | 154275 | |
| <I/σ(I)> | 6.43 | |
| Completeness [%] | 94.3 | |
| Redundancy | 2.29 | |
| CC(1/2) | 0.991 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG4000, 50mM MgCl2, 100mM HEPES pH7.5 |
