6FO8
Vitamin D nuclear receptor complex 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0721 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 66.690, 66.690, 264.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.160 - 2.300 |
| Rwork | 0.198 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hc4 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16402 | |
| <I/σ(I)> | 28.08 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 8 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | BisTris pH 6.5, 1.6 M lithium sulfate and 50 mM magnesium sulfate |
