6FN5
Crystal structure of ERK2 in complex with an adenosine derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.425, 69.888, 59.452 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 23.292 - 1.932 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qyz |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.298 | 28.595 | 2.040 |
| High resolution limit [Å] | 1.932 | 6.110 | 1.932 |
| Rmerge | 0.061 | 0.017 | 0.525 |
| Rmeas | 0.085 | 0.028 | 0.726 |
| Rpim | 0.052 | 0.018 | 0.423 |
| Total number of observations | 72769 | 2159 | 9067 |
| Number of reflections | 27188 | 892 | 3382 |
| <I/σ(I)> | 8.4 | 29.5 | 1 |
| Completeness [%] | 94.7 | 94.7 | 80.8 |
| Redundancy | 2.7 | 2.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG MME 2000, 0.1M MES pH 6.5, 0.1M ammonium sulfate, 0.02M beta-mercaptoethanol, 0.002M magnesium sulfate |
