6FMQ
Keap1 - peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2017-11-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.652, 76.129, 211.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.837 - 2.100 |
| R-factor | 0.1671 |
| Rwork | 0.165 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vng |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.203 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | |
| Number of reflections | 71681 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 4.0 M ammonium acetate, 0.1 M sodium acetate trihydrate pH 4.6 |
