6FM0

Deoxyguanylosuccinate synthase (DgsS) and ATP structure at 1.7 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978570
Spacegroup name	C 1 2 1
Unit cell lengths	158.860, 60.410, 91.030
Unit cell angles	90.00, 114.76, 90.00

Refinement procedure

Resolution	45.600 - 1.700
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.600	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	884293
<I/σ(I)>	11.65
Completeness [%]	97.1
Redundancy	4.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG3350 0.2M Na2SO4