6FLJ

Crystal structure of Cep120 C2A_K76A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	40.842, 51.367, 66.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.710 - 1.751
R-factor	0.2117
Rwork	0.210
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4icw
RMSD bond length	0.012
RMSD bond angle	1.243
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2863: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.710	1.814
High resolution limit [Å]	1.750	1.751
Rmerge	0.069	1.925
Rmeas	0.072
Rpim	0.020	0.567
Number of reflections	14678	1425
<I/σ(I)>	24.44	1.29
Completeness [%]	99.9	99.65
Redundancy	12.7	12.2
CC(1/2)	1.000	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350 and 0.2 M NaSCN