6FLF
Deoxyguanylosuccinate synthase (DgsS) structure at 1.33 Angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.920, 59.480, 73.980 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 49.400 - 1.330 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 1.410 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 88372 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.2 | |
| Redundancy | 4.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2M NaCl 12% PEG 6K |
