6FL4
A. thaliana NUDT1 in complex with 8-oxo-dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.07 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.898, 77.412, 122.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.000 - 1.600 |
| R-factor | 0.18052 |
| Rwork | 0.179 |
| R-free | 0.21384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 47078 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 98.4 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 40 % (v/v) PEG300, 0.1 M sodium acetate/acetic acid (pH 4.5), 0.2 M NaCl |
