6FKW
Europium-containing methanol dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9773 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.080, 101.180, 107.790 |
| Unit cell angles | 115.21, 97.53, 91.78 |
Refinement procedure
| Resolution | 96.270 - 1.400 |
| R-factor | 0.16939 |
| Rwork | 0.169 |
| R-free | 0.18484 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4mae |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.027 | 0.750 |
| Number of reflections | 352259 | |
| <I/σ(I)> | 11.5 | 1 |
| Completeness [%] | 90.5 | 87 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% w/v PEG 8000, 0.3 M NaCl |
