6FKQ

THE CRYSTAL STRUCTURE OF A FRAGMENT OF NETRIN-1 IN COMPLEX WITH A FRAGMENT OF DRAXIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 64 2 2
Unit cell lengths	130.794, 130.794, 183.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.010 - 3.070
R-factor	0.237
Rwork	0.235
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4urt
RMSD bond length	0.012
RMSD bond angle	1.881
Data reduction software	MOSFLM
Data scaling software	Aimless (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.130	3.230
High resolution limit [Å]	3.070	3.070
Rmerge	0.587	2.897
Number of reflections	18063
<I/σ(I)>	8.7
Completeness [%]	99.9	99.7
Redundancy	32.9	18.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	298	1.6 M AMMONIUM SULPHATE AND 0.1 M SODIUM CITRATE, AT PH 4 TO PH 5, VAPOR DIFFUSION, TEMPERATURE 298K