6FKN

Drosophila Plexin A in complex with Semaphorin 1b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0719
Spacegroup name	P 65 2 2
Unit cell lengths	153.630, 153.630, 425.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	133.047 - 4.801
R-factor	0.2854
Rwork	0.285
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kfm
RMSD bond length	0.005
RMSD bond angle	0.811
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	133.047	4.970
High resolution limit [Å]	4.801	4.801
Rmerge	0.339
Number of reflections	15309
<I/σ(I)>	7.41
Completeness [%]	99.9
Redundancy	20.9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES and 12% (w/v) PEG 20 000