6FKK

Drosophila Semaphorin 1b, extracellular domains 1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 61 2 2
Unit cell lengths	163.256, 163.256, 140.403
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	81.628 - 2.780
R-factor	0.2082
Rwork	0.206
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oky
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.628	2.880
High resolution limit [Å]	2.780	2.780
Rmerge	0.117	1.641
Number of reflections	27778
<I/σ(I)>	10.93
Completeness [%]	96.7
Redundancy	11.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M trisodium citrate and 20% (w/v) PEG 3350