6FK6

Crystal structure of N2C/D282C stabilized opsin bound to RS01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-21
Detector	PSI PILATUS 6M
Wavelength(s)	1.000020
Spacegroup name	H 3 2
Unit cell lengths	244.209, 244.209, 111.752
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.790 - 2.360
R-factor	0.216
Rwork	0.216
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INHOUSE MODEL
RMSD bond length	0.004
RMSD bond angle	0.772
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.790	2.420
High resolution limit [Å]	2.360	2.360
Rmerge	0.114	5.370
Number of reflections	52217
<I/σ(I)>	15.39	0.55
Completeness [%]	100.0	100
Redundancy	11.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	AMMONIUM SULPHATE, SODIUM ACETATE, D(+)-TREHALOSE, PH 6.0