6FJM

tubulin-Disorazole Z complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	104.390, 157.630, 179.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.974 - 2.100
R-factor	0.1807
Rwork	0.179
R-free	0.21290
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.004
RMSD bond angle	0.621
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2863: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.974	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.105	3.126
Rmeas	0.107	3.354
Number of reflections	172635
<I/σ(I)>	18.5	0.68
Completeness [%]	100.0	99.8
Redundancy	12.1	7.9
CC(1/2)	0.999	0.243

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	294	10 % PEG 4k, 16 % glycerol, 30 mM MgCl2, 30 mM CaCl2, and 100 mM 2-(N-morpholino)ethanesulfonic acid/imidazole