6FJF
Tubulin-FcMaytansine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00036 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.373, 156.739, 180.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.547 - 2.402 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.25750 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2863: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.216 | 2.586 |
| Rmeas | 0.234 | 2.814 |
| Number of reflections | 116072 | 8494 |
| <I/σ(I)> | 9.96 | 0.75 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.994 | 0.277 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 294 | 10 % PEG 4k, 16 % glycerol, 30 mM MgCl2, 30 mM CaCl2, and 100 mM 2-(N-morpholino)ethanesulfonic acid/imidazole |
