6FJ7
Caldiarchaeum Subterraneum Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.450, 42.800, 51.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.890 - 1.050 |
| R-factor | 0.15544 |
| Rwork | 0.153 |
| R-free | 0.19512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.337 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.900 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.051 | 0.627 |
| Number of reflections | 30589 | 4406 |
| <I/σ(I)> | 22.39 | 2.18 |
| Completeness [%] | 98.2 | 89 |
| Redundancy | 11 | 5.1 |
| CC(1/2) | 0.999 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium acetate pH 4.5, 200 mM lithium sulfate, 50% PEG 400 |
