6FIH
Crystal structure of the ANX2 ectodomain from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-08 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999894 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.566, 41.766, 93.371 |
Unit cell angles | 90.00, 117.37, 90.00 |
Refinement procedure
Resolution | 44.290 - 1.080 |
R-factor | 0.16029 |
Rwork | 0.159 |
R-free | 0.18269 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.714 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.290 | 1.150 |
High resolution limit [Å] | 1.080 | 1.080 |
Rmeas | 0.040 | 1.290 |
Number of reflections | 175203 | 25949 |
<I/σ(I)> | 18.94 | 1.17 |
Completeness [%] | 96.4 | 88.7 |
Redundancy | 6.46 | 5.5 |
CC(1/2) | 1.000 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 28% PEG 3350 |