6FIG
Crystal structure of the ANX1 ectodomain from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.991876 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.991, 68.745, 70.046 |
| Unit cell angles | 88.54, 74.93, 71.74 |
Refinement procedure
| Resolution | 47.270 - 1.480 |
| R-factor | 0.17221 |
| Rwork | 0.171 |
| R-free | 0.19214 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.482 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.270 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmeas | 0.060 | 2.170 |
| Number of reflections | 151428 | 10966 |
| <I/σ(I)> | 17.93 | 1.2 |
| Completeness [%] | 98.4 | 95.6 |
| Redundancy | 10.3 | 10.1 |
| CC(1/2) | 1.000 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 27.5% PEG 3350, 0.1M HEPES pH 7.5 |
