6FIA
Structure of the human LINE-1 ORF1p coiled coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.160, 250.610, 33.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 125.310 - 2.650 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ykp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.310 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 23830 | 1688 |
| <I/σ(I)> | 14.9 | 2.2 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M HEPES, 0.15M AMMONIUM SULFATE, 12% PEG 2000 |
