6FI0
Crystal structure of BAZ2A PHD zinc finger in complex with Fr 19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.095, 72.095, 99.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.340 - 1.900 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.23101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qf2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.898 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.340 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | |
| Number of reflections | 21069 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 2.2-2.4 M sodium/potassium phosphate |
